Evaluation of sphingolipid metabolism in renal cortex of rats. Myocyte death in streptozotocininduced diabetes in rats. Stz induced increased glycemia in diabetic rats vs control rats. Novel insight into streptozotocininduced diabetic rats. Induction of diabetes diabetes was induced by single tail vein injection of stz 45 mgkg sigma, st. Daily treatment of stzinduced diabetic rats with an. Currently, stz is most often used to induce diabetes in rats and mice. Markers of diabetes side effects can bemonitored upon request. After 10 days of biotelemetry data collection, diabetes was induced in five of the 10 rats by a single intraperitoneal injection of stz 60 mg kg body weight. The rats in this model were divided into four groups. A comparison with the chronic changes in human diabetes will indicate whether the stzdiabetic rat is an adequate model of the human disease. Oxidative stress and antioxidant enzyme changes in the stzinduced dm rat group. Effects of stzinduced diabetes on the relative weights of.
Moreover, diabetic changes in rats with stznainduced diabetes are not fully characterized. Male mice and rats tend to be more susceptible to stzinduced diabetes 10, 15, 16, 2225. The full text of this article is available as a pdf 180k. Materials and methods intraperitonial injection of 40mgkg dose of streptozotocin in adult wistar rats, makes pancreas swell and at last causes degeneration in langerhans islet beta cells and. Beneficial effects of coenzyme q10 in streptozotocin. Antidiabetic and hypolipidaemic properties of garlic. Deterioration of bone quality by streptozotocin stz induced type 2 diabetes mellitus in rats. Propel your preclinical research using jax inventoried, ondemand, and unique stzinjected models generated on various genetic backgrounds, as well as additional services. Key words streptozotocin, glucose levels, induced diabetic, bodyweight. Pdf induction of diabetes by streptozotocin in rats. Materials and methods intraperitonial injection of 40mgkg dose of streptozotocin in adult wistar rats, makes pancreas swell and at last causes degeneration in langerhans islet beta cells and induces experimental diabetes mellitus in the 4872 hours.
Stz induced diabetes has antihyperglycemic and antidyslipidaemic action or not. When treated with stz, the animals developed severe hyperglycaemia, which differed substantially from the levels of the control rats. Metformin treatment significantly reduced blood glucose in stz induced diabetic mice compared with stz group p in the body weight between metformintreated and szt group, though the body weight was significantly decreased after injection of stz p stz induced diabetes streptozotocin stz is an alkylating agent that targets insulinproducing beta cells in the pancreas, mimicking type 1 diabetes phenotypes in mice. Animals showing glucosuria more than 2% diastix, bayer diagnostics, india or blood glucose level 140 mgdl 48 h after stz injection were selected for the experiment. Ultraperformance liquid chromatographymass spectrometry uplcms and univariatemultivariate data analysis methods were applied to determine differences in the serum metabolomes associated with stz induced dm and teta treatment. Streptozotocin stz is a widely used chemical for the induction of experimental diabetes in rodents. Research open access metabolomic analysis of rat serum in.
Rats treated with stz and na manifest symptoms of type 2 diabetes, while animals with stzinduced type. Rodents also show a substantial gender difference in stz sensitivity. However, another important question centers around whether the hfdstz rat mimics an early or late stage of type 2 diabetes. Induction of diabetes by streptozotocin in rats ncbi nih. Pdf the objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of. Induction of diabetes is monitored by routine tests blood levels of glucose and glycosylated hemoglobinhba1c. Compared to normal nondiabetic rats, stzinduced diabetic rats had approximately 200% higher serum glucose, 50% higher serum cholesterol and 30% higher serum triglyceride levels as well as 86% higher urinary protein levels.
A comparison with the chronic changes in human diabetes will indicate whether the stz diabetic rat is an adequate model of the human disease. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. Effects of stz induced diabetes on the relative weights of kidney, liver and pancreas in albino rats. The stz was dissolved in a citrate buffer solution 0. This study investigated the role of heme oxygenase ho1 in the cardiac tissue injury of acute ischemiareperfusion ir in diabetic streptozotocin stz induced hyperglycemic rats. Guangyi liu, fei han, yi yang, yi xie, hong jiang, youying mao, huiping wang, minmin wang, rongjun chen, jun yang, jianghua chen, evaluation of sphingolipid metabolism in renal cortex of rats with streptozotocininduced diabetes and the effects of rapamycin, nephrology dialysis transplantation, volume 26, issue 5, may 2011, pages 14931502.
To determine whether myocyte death and angiotensin ii at ii formation are implicated in the development of diabetic cardiomyopathy, rats were injected with streptozotocin, and apoptosis and. Streptozotocininduced type 1 diabetes in rodents as a model. Streptozotocininduced diabetic models in mice and rats. Streptozotocin or streptozocin inn, usp stz is a naturally occurring alkylating antineoplastic agent that is particularly toxic to the insulinproducing beta cells of the pancreas in mammals. To understand the pathophysiological mechanisms and factors involved in diabetes, the use of pancreatic regeneration studies is increasing in an attempt to understand the behavior of pancreatic beta cells. Streptozotocin use in mice and rats version 1 date of publication. The animals with blood glucose above 250 mgdl were randomized in different groups. The effect of streptozotocininduced diabetes in rats. Animals were treated with escin at a dose of 5, 10 and 20 mgkg after six weeks of diabetes induction for the next four weeks. In this experimental model, the severity of diabetes is different depending on doses of stz and na.
Research open access metabolomic analysis of rat serum. The animals then receive injections of streptozotocin to induce insulindependent diabetes mellitus. Streptozotocin stz is a naturally occurring nitrosourea with molecular weight of 265 and empirical formula of c14 h27 n5 o1211. Streptozotocininduced diabetes in the rat is associated with. Methods diabetes was induced in 8 week old male wistar rats n96 using a single rapid injection. Antihyperglycemic and antidyslipidemic potential of. Myocyte death in streptozotocininduced diabetes in rats is. Intravenous injection of 60mgkg dose of streptozotocin in adult wistar rats, makes pancreas swell. Ultraperformance liquid chromatographymass spectrometry uplcms and univariatemultivariate data analysis methods were applied to determine differences in the serum metabolomes associated with stzinduced dm and teta treatment. Detailed in this unit are protocols for producing stz. In rats, streptozotocin stz induces diabetes when used at doses ranging from 45 to 70 mgkg rakieten et al. Three days after degeneration of beta cells, diabetes was induced in all animals. In the untreated diabetic group a significant decrease in the total body weight 47% was observed fig.
Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with nonketosis hyperglycemia in some. Propel your preclinical research using jax inventoried, ondemand, and unique stz injected models generated on various genetic backgrounds, as well as additional services. Sep 14, 2017 experimental model of stz induced diabetes. Therefore, four groups of rats were allocated as control c, stz induced dm d, and stz induced dm supplemented with 300 mgkg bw of either aqueous extract we or ethanolic extract ee of a. Isometric tension responses to the vasoconstrictors phenylephrine, serotonin and pgf 2. Relationship between cardiovascular dysfunction and. We studied the effects of streptozotocin stz induced diabetes on the body weights of animals and the relative. Metformin ameliorates insulitis in stz induced diabetic mice. Changes in oxidative stress and antioxidant enzyme. Novel insight into streptozotocininduced diabetic rats from. Modeling type 2 diabetes in rats using high fat diet and. Stz induced type 2 diabetic rats with or without insulin therapy. Described in this unit are two protocols used to produce stz induced diabetes in mice basicprotocol1andratsbasicprotocol2. Pdf induction of diabetes by streptozotocin in rats researchgate.
Compared to normal nondiabetic rats, stz induced diabetic rats had approximately 200% higher serum glucose, 50% higher serum cholesterol and 30% higher serum triglyceride levels as well as 86% higher urinary protein levels. Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with nonketosis hyperglycemia in some animal species. Antidiabetic effect of betulinic acid on streptozotocin. Streptozotocininduced experimental diabetes in male wistar rats. Streptozotocin stz is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus t1dm. The rat mouse model of diabetes mellitus induced by streptozotocin injections is one of the most widely used model of type i diabetes and mimics human pathological situation of decreased insulin production untreated type i diabetes or decrease tissue responsiveness to insulin. Metformin treatment significantly reduced blood glucose in stzinduced diabetic mice compared with stz group p ratsmice stz model advantages. Induction of diabetes by streptozotocin in rats article pdf available in indian journal of clinical biochemistry 22222. Longterm streptozotocininduced diabetes in rats leads to. The observed results showed that pb considerably reduced the proinflammatory cytokine interleukin. The plasma levels of glucose, tg, tc, ldlc and vldlc, mda, and bilirubin and the activities of transaminases and gr were significantly. Diabetes is associated with retinal microvascular changes that include leakage, increased neovascularity, and increased tortuosity, as well as neutrophil attachment and leukostasis. The dosage of this antidiabetic effect was yet to be explored.
Collectively, the results show that stzinduced diabetes results in a decrease in blood pressure and that insulin treatment can restore blood. Antidiabetic and hypolipidaemic properties of garlic allium. Effect of streptozotocin on glucose levels in albino wister rats. Changes in oxidative stress and antioxidant enzyme activities. Antidiabetic and antihyperlipidemic activity of euphorbia. In the rat models of stzinduced diabetes, male rats at 8 weeks of age 200250 g are starved for 16 h and injected once into the tail vein with stz sd 55 mgkg, wky 60 mgkg, shr 45 mgkg in sodium citrate buffer 1 mlkg cooper et al. However, the effects of diabetes on genital tissue structure, innervation and function remains poorly characterized. Glucose levels were higher in the diabetic control dmc rats than in the control, gbc, mdc100, and mdc200 rats. The streptozotocin stz induced model of diabetes in rats serves as a validated and reproducible model of retinal hypervascularity in brown norway rats. Diabetes mellitus, often simply referred to as diabetes.
The final symptoms of insulin deficiency are clearly seen in rats afflicted with diabetes chemically by streptozotocin. To examine the effect of the variation in the dosage of c. Centella asiatica has been used for healing diabetes mellitus. Rats with diabetes induced by streptozotocin stz and nicotinamide na are often used in animal studies concerning various aspects of diabetes.
Antidiabetic and antihyperlipidemic effect of duvalia. Effect of induceddiabetes on body weight stzinduced diabetic rats were kept with continuous sugar monitoring before having their diet supplemented with wp for five weeks. Pdf antidiabetic effect of centella asiatica extract. Effect of streptozotocin on glucose levels in albino. Metabolic disturbances and defects in insulin secretion in. Treatment with glibanclamide and extracts of et were p diabetes was induced in rats with streptozotocin 55 mgkg. Alhagi maurorum camel thorn plant is a promising medicinal plant due to the presence of flavonoids and phenolic compounds as major contents of its constituents. Luis, mo, usa to male sprague dawley rats 200260 g. Induction of diabetes by streptozotocin in rats springerlink. Therefore, four groups of rats were allocated as control c, stzinduced dm d, and. Stzinduced type 2 diabetic rats with or without insulin therapy. It is used in medicine for treating certain cancers of the islets of langerhans and used in medical research to produce an animal model for. We studied the effects of streptozotocin stzinduced diabetes on the body weights of animals and the relative. The effects of 1 hemin, an inducer of ho expression and activity, and 2 zinc protoporphyrin ix znppix, an inhibitor of ho activity, have also been investigated on the tissue injury by ir and some.
Effects of stzinduced diabetes on the relative weights of kidney, liver and pancreas in albino rats. In stz treated dm rats, mrna levels and enzyme activities of ar significantly increased in blood sera and brains of dm rats at week 16 following stz induced dm fig. Effect of induced diabetes on body weight stz induced diabetic rats were kept with continuous sugar monitoring before having their diet supplemented with wp for five weeks. In control and streptozotocintreated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase i, endothelial nitric oxide synthase enos and cgmp. Streptozotocininduced type 1 diabetes in rodents as a. Deterioration of bone quality by streptozotocin stzinduced type 2 diabetes mellitus in rats. To develop a standard stz model of diabetes to test a drug candidate, we suggest using 810 animals per each. Streptozotocininduced diabetes in laboratory animals presents an experimental model whose value is in the elucidation of causal relationships related to human diabetes mellitus. Streptozotocininduced diabetes as a model of diabetic retinopathy in brown norway rats robin dean, robert sukhu, leslie nemeth, qin zhang, isaac hakim, ali. Streptozotocininduced diabetic ratsmice stz model advantages.
696 40 223 196 199 1236 611 996 1326 314 1606 523 474 875 933 996 779 1586 1322 21 1420 967 277 942 477 484 1542 75 440 843 1295 1014 359 1087 1343 922 1274 1354 1162 435 38 844 890 632 1074 920 806 593 846 683 535